الفهرس 
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Abstract
In the present study, two groups (I and II) of animals were tested. Group I composed of 170 tuberculin reactor cattle and out of them 88 animals (51.8%) were positive to bTB by microscopic examination of direct smears while by culture on L-J medium (4% pyruvate) M.bovis was isolated from 114 animals (67.1%). The bacteriological examination of tissue samples collected from different organs showing tuberculous lesions from group I (170 lymph node, 40 lung, 34 liver and 20 spleen) revealed that by microscopic examination spleen showed the highest percentage of positive results, 55% (11/20), followed by lung 52.5% (21/40) then lymph nodes 51.8% (88/170) and finally liver at a percentage of 50% (17/34), also by culture method Spleen had the highest percent of positive results, 70% (14/20), followed by lung, lymph node and liver in a percentage of 67.5% (27/40), 67.1% (114/170) and 61.8% (21/34) respectively. On the other hand, group II composed of 200 animals which were subjected to SICTT on two phases, out of them 26 animals were positive reactors, 14 from the first phase and 12 from the second one, of them 19 (73.1%) animals were positive to bTB by culture on L-J (4% pyruvate). Serum samples were collected from group II, before being tested by SICTT, to be tested by ELISA and results revealed that out of the total 200 animals, 20 (10%) animals were positive to bTB when using PPD-B as coating antigen compared to 23 (11.5%) positive animals by using a commercial mixture antigen. The use of IFN-γ assay, on heparinized whole blood samples collected from the same group before SICTT, detected 26 positive animals out of the total 200 animals at a percentage of (13%). By comparing results of the IFN-γ assay to results of the first phase SICTT, it appears that the IFN-γ assay could detect 12 positive cases that tested negative by the first phase SICTT, of them 9 animals were confirmed to be positive by the culture method. It can be concluded that the use of mixture antigen in ELISA helps giving more efficient diagnosis than using PPD-B antigen. Also it can be added that the IFN-γ assay detected more positive cases than other tests which indicates that it appears to be more sensitive than both SICTT and ELISA and should be used in parallel to SICTT to allow detection of more positive animals before they become source of infection to other animals and human.