الفهرس | Only 14 pages are availabe for public view |
Abstract A taxonomical study was carried to assess the inter- and intraspecific variation among 34 OTU’s of two Marrubium species distributed along the western Mediterranean coastal region of Egypt; M. alysson L. and M. vulgare L. Four approaches were suggested to discriminate among the studied OTU’s; morphological characters, molecular markers (seed protein electrophoresis and RAPD analyses), vegetation characteristics, land use types and soil analysis as well as allelopathy as a biochemical marker. The constructed dendrograms by using the different types of characters separately or collectively are congruent in the segregation of M. vulgare. By means of seed protein electrophoresis, M. vulgare is characterized by one species-specific band at 24.07 KD. However, M. alysson is acquired by four speciesspecific at 20, 29.14, 47.55 and 52.13 KD. Within the studied OTU’s of M. alysson, four distinct samples are recognized; each is characterized by particular total number of bands and percentage of polymorphism. Random Amplified Polymorphic DNA Analysis (RAPD) is performed through six operon primers which produce 221 bands, of which 175 bands are polymorphic. Both M. vulgare and OTU 31 of M. alysson are segregated together in the dendrogram. Therefore, the present study recommends that OTU 31 of M. alysson can be allocated at higher rank than biotype. The present investigation indicates that there is a strong association between the three biotypes within M. alysson using different taxonomic markers and both geographical and edaphic factors which strongly influence the evolution of these populations. The existence of great environmental changes and high intensities of human. |