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العنوان
Molecular And Epidemiological Studies Of Salmonellosis In Poultry And Man /
المؤلف
Hussein, Mona Kadry.
هيئة الاعداد
باحث / منى قدري حسين
مشرف / ناهد حامد غنيم
مشرف / وفاء وحيد محمد رضا
الموضوع
Salmonella. Poultry Meat Quality Symposium. Enterotoxin.
تاريخ النشر
2015.
عدد الصفحات
130 P. :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
البيطري
تاريخ الإجازة
1/1/2015
مكان الإجازة
جامعة القاهرة - كلية الطب البيطري - Zoonoses
الفهرس
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Abstract

This work was carried out to study the occurrence of salmonella infections in chicken in different poultry shops and farms in Egypt. A total of 619 chicken samples including 349 chicken meat samples and 270 chicken cloacal swaps and 9 water samples collected from water tanks during slaughtering process. Also 394 egg samples collected from different localities in addition to 230 human stool specimens were collected. All samples were subjected to laboratory examination under a septic conditions for bacteriological examination which revealed that 14 (4.01%) chicken meat samples, 8 (2.96%) chicken cloacal swaps, 2 (22.2%) water samples, 2 (0.5%) egg samples and 6 human stool specimens (2.6%) were positive for Salmonella species and the isolates which preliminary identified biochemically as salmonella bacteria were subjected to serological identification [ (Salmonella Enteritidis, Salmonella Infantis, Salmonella Kentucky, Salmonella Montevideo, Salmonella Poona, Salmonella Rubislaw, , Salmonella Typhi, Salmonella Typhimurium, and Salmonella Virginia) in chicken meat samples (Salmonella Haifa and Salmonella pullorum) in chicken cloacal swaps (Salmonella Enteritidis and Salmonella Typhimurium) in egg samples and (Salmonella Enteritidis, Salmonella Haifa, Salmonella Kentucky, Salmonella Typhimurium and Salmonella Virchow) in human stool specimens]. Agar disc diffusion method for all 30 salmonella isolates revealed that 9 (30%) salmonella isolates have drug resistance. Also, genotyping showed that (64.28%) of chicken meat salmonella isolates was positive for stn gene, (37.5%) in chicken cloacal swaps isolates and (100%) were positive in both egg and human isolates from them 86.6% of salmonella isolates were positive for Enterotoxigenicity by infant mouse assay. Sequencing and phylogentic tree of 9 isolates included in this study and 16 isolates retrieved from gene bank resulted in assessment the relations between different isolation sources. The results proved that under cooked poultry meat and egg might pose a public health problem for humans also, appropriate measures must be taken to control the spread of resistant bacteria to human.