الفهرس 
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Abstract
The study was conducted on 120 Egyptian buffaloe cows raised in a large governmental farm located in the valley of AL-sheh ,Assiut. These animals were healthy. Their age ranged from 3-8 years and their parity was (3-7). After examination rectally and by using ultrasonography, a total of 76 animals With a history of post partum anoestrus (≥4 months) and with no palpable structures on their ovaries were included in the study. All animals were treated by Exapar and Janova combination according to the manufacturer’s instructions as Comprise of oral Exapar (E); 100 ml, for 5 days followed by oral Janova (J) 3 capsules for 6 days (days 1-2, 6-7 and 17-18). Milk samples were collected every four day start from 0 day before treatment till 32 days after treatment. centrifugation (3000 rpm for 15 min.), 100 μl of 0.1% sodium azide was added to 5 ml of milk sample as a preservative according Van de Wiel and Koops1986 . Samples were stored at –20°C until they were analyzed for MPL, using Specific test kit (progesterone ELISA kit).Transrectal ultrasonography was done for each buffaloe cow during the postpartum period using a Color-Doppler ultrasonographic examinations were done. Buffaloe cows with history of long post-partum anestrus more than 4 month. The animals were examined (n=120) per rectum and monitored ultrasonographically, after examination a total of 76 animals were suffered from anestrous. When all medicated anestrous buffaloes scanned ultrasonographically with an interval of 4-5 days we noticed that when the animal had a large follicle, the rate of response was 18 % (9 out of 50) and these animals showed heat after 5 days from treatment. However (22 out of 50) showed heat after 9-13 days when the ovaries contained medium sized follicles. In addition when the ovaries contained small sized follicles (19 out of 50) buffaloes (38 %) expressed heat within 18-32 days of treatment. The milk progesterone levels were decreased after treatment when compared with their levels before treatment.