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العنوان
Studies On Some Gastrointestinal Diseases Of Dogs /
المؤلف
Abdel-Hakim, Khaled Mohammed.
هيئة الاعداد
باحث / خالد محمد عبد الحكيم
مشرف / رفعت محمد حسين عرب
مشرف / أبو زيد عبد المجيد أبو زيد
مشرف / أحمد سمير محمد شحاتة
الموضوع
Dogs. Enterobacteriaceae. Shigella. E. coli infections. Salmonella.
تاريخ النشر
2015.
عدد الصفحات
178 P. :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
البيطري
تاريخ الإجازة
1/1/2015
مكان الإجازة
جامعة القاهرة - كلية الطب البيطري - Internal Medicine and Infectious diseases
الفهرس
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Abstract

In this study a total of 717 samples from dogs and their environment from eight different localities were collected. All the samples were subjected to different investigations for isolation and identification of Enterobacteriaceae. 707 Enterobacteriacae isolates could be recovered (616/536 living dogs , 41/ 18 died dogs , 60/ 91 enviromental samples). Epizootiolgical studies revealed that the incidence of Enterobacreiaceae was higher in stray and clinic dogs but lower among kenneled dogs, while the incidence was higher in puppies than adult dogs. The isolation rate in diseased dogs suffered from intestinal disorders was higher in correlation to apparently healthy dogs. E.coli (62%) and Proteus species (20.8%) were the most predominant types. Salmonella could be isolated from one and half year apparently healthy police dog recently imported from Netherlands identified as S.kiel and confirmed to have invA gene by PCR. According to available knowledge, it was the first time to isolate S.kiel strain from dogs in Egypt. Experimental infection of dogs with the isolated S.kiel serotype was done and all the clinical, microbiological, molecular and histopathological results were recorded. from 536 living dogs used in this study, 337(62.8%) E. coli strains were detected. Enteropathogenic O26 k60 E. coli strain containing eae gene was isolated from all the internal organs of died one and half year old police German shepherd dog and proved experimentally through all its clinical, microbiological, molecular and histopathological results that the E. coli O26K60 was the possible cause of death. So concluded that E. coli may be implicated in sever gastrointestinal disease and may lead to death even in adult dogs. By using PCR technique , randomly selected 50 E. coli strains, 31(62%) were confirmed to be positive for eae gene .Its incidence was higher in diseased dogs (75%) and dead dogs (80%) than healthy ones (40%). The efficiency of PCR for the studied strains indicates that this technique can be recommended for the diagnosis of Salmonella and enteropathogenic Escherichia coli as a differential from other pathogens causing diarrhea. Shigellae could be isolated from fecal samples of a diseased stray dog and one apparently healthy kenneled dog. Studying the in vitro sensitivity of Salmonella, Shigella and O26K60 E. coli isolates to 10 antibacterial agents revealed that all isolates were highly sensitive to ciprofloxacin and chloramphenicol, while S.kiel and Shigella were resistant to tetracycline and E. coli O26K60 were resistant to ampicilin.