الفهرس 
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Abstract
The aim of this study was to investigate the toxic effects of aflatoxin B1 on Nile tilapia Oreochromis niloticus and attempt to detoxify these drastic effects by using vitamin E and coumarin and clay supplementation in diets. Three hundred and sixty fingerlings healthy fingerlings of Nile tilapia (±7.3 g /fish) were randomly distributed into 24 glass aquaria(75 L), representing 8 treatments all fish were divided into two main groups. The first main group was given basal diet without aflatoxin B1, and the second main group was given basal diets containing 0.25 ppm aflatoxin B1. Within each the previous two main groups they were divided to other 4 subgroups; the first group fed diet without any treatment (control), the second group was treated with coumarin (1, 2 benzopyrone) supplemented (5 g/kg diet) in fish diets, the third group was treated with vitamin E supplemented (50 mg/kg diet) in fish diets and the fourth fed the same diet and supplemented with clay 20 g/ kg bentonite feed. Ranges of water quality parameters within the acceptable ranges required for normal growth of tilapia, The experimental period lasted 98 days. Live body weight and daily gain were reduced by 9.10 and 11.36% in fish fed diets contaminated with aflatoxin B1, when compared with those fed diet without aflatoxin B1, also, feed conversion was impaired by 14.91%. Clay treatment recorded higher final body weight and daily gain followed by the fish group fed diet supplemented with coumarin and vitamin E., than that of the control treatment. The best feed conversion was obtained in same order in fish group treated with clay, coumarin and vitamin E. Serum total protein and albumin decreased, while serum ALT, AST, urea-N and Createnine increased in fish groups fed diets contaminated with aflatoxin B1. Serum total protein increased more in fish groups fed diets supplemented with coumarin,clay and vitamin E than the control group. Residual of aflatoxin reduced by 95.88% in fish body fed the control diet compared with those fed diet contaminated with aflatoxin B1. Fish group treated with clay ,coumarin and vitamin E decreased the aflatoxin by 95.21 , 92.99, 91.86%, respectively when compared with the control group. Fish groups fed diets contaminated with aflatoxin recorded lower return from body gain and final margin by 11.35 and 25.95%, respectively, when compared with the control group. The return from body gain increased by 26.09 ,26.09 and 15.94%, respectively, in fish fed diets supplemented with clay, coumarin and vitamin E when compared with fish group fed diet without feed additives. It could be recommended for using vitamin E, coumarin or clay treatments to alleviate the toxic effects of aflatoxin B1 contaminated diets.