الفهرس | Only 14 pages are availabe for public view |
Abstract Tests for probiotic criteria of 7 bacilli and 4 cocci were obtained and purified. These isolates were found to be Gram positive, catalase negative, non-spore-formers and non-motile which meets the basic characteristics of some probiotic bacteria. The three isolates with high acid and bile salt tolerance since they grew well in media with pH 3 and contained bile salt at concentration up to 0.3%. and strong adhesion to epithelial cells of sheep intestine. The stability of selected isolates in media contained sodium chloride (NaCl) 8%, 13 bacterial isolates. Proved to be highly tolerant to the tested salt concentration. Out of 10 isolates, 7 were able to stay alive after exposure to a temperature of 50 C. for 20 minutes. All of the tested isolates were able to coagulate the litmus skim milk solutions after 10 hours of incubation. Among the 7 tested isolates Lactobacillus no. 9 isolated from homemade pickled cucumber showed maximum zone of inhibition i.e. 14, 15, 16 and 18 mm in diameter against Staphylococcus aureus, Escherichia coli, Pseudomonas sp and Bacillus cereus respectively. Among the 6 tested isolates Lactobacillus no. 1 isolated from homemade Laban zee (L1Lz) showed maximum inhibition against the aflatoxin producing fungi i.e. 51.1 and 48.4 % growth inhibition against Aspergillus flavus and Aspergillus parasiticus respectively. The results also showed that the viability of Lactobacillus isolate from (L3Lz) showed significantly better survival when stored in freezer for 11 weeks after which viability gradually decreased to be 0.0 at the 14th week. |