الفهرس 
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Abstract
This work was conducted to study the role of actinomycete in removal of polluting chlorinated hydrocarbons through the assessment of their degradation abilities in microcosm experiment. In addition to the study of the abilities of selected actinomycete isolates immobilized on activated carbons for pollutants removal.
Six localities along the Suez freshwater canal were
studied; these are Suez water station, Amir Village, Al
Shalofa, Kobry El saal, Sarabium water station and Abu
Atwa. The characterization of water included physical,
chemical and microbiological analysis besides isolation of
actinomycetes from the canal sediments using enrichment
technique.
The main experimental results can be summarized as the following:
- The water characteristics revealed that the water quality deterioration especially in the Amir village which may be due to industrial and domestic wastewater from human and agriculture activities. The microbiological analysis showed that all investigated sites were suffering from pollution with the untreated sewage and animal wastewater
- The isolation of actinomycetes from Suez freshwater canal sediments by enrichment culture technique resulted in
Summary
۱۳۸
two isolates using DDT enrichment (N1 and N2), while no actinomycetes was observed using PCP enrichment.
- The actinomycete isolates (St. S7, St. S11, St. S40, St. S85 and St. N1) showed comparably highest degradation abilities. Their identification was confirmed by phylogenetic analysis and they were selected for further investigation in the present study.
- The immobilization of actinomycete strains St. lavendulocolor (S40) and St. capoamus (N1) on carbon material could significantly enhance the actinomycetes degradation abilities, so this technology can be widely accepted for the treatment of polluted water effluents.
- The use of agriculture wastes for the preparation of activated carbon with high adsorption performance is very attractive from the point of view of their contribution to decrease the cost of waste disposal, therefore helping environmental protection
- The biofilm formed due to combination of actinomycetes consortium and microorganisms present in sand enhanced the removal of pollutants.
- Purification of the peroxidase and laccase enzymes of the studied isolates is envisaged for our future work, which would be of much significance since the production of all three major chlorinated hydrocarbon-degrading enzymes by
Summary
۱۳۹
St.viridochromogenes S40 could be consider as a novel character among actinomycetes.