الفهرس 
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Abstract
A total of 88 Pasteurella multocida isolates were isolated from 256 samples (34.4%). Dead calves showed the highest percentage of P. multocida isolation (80%), followed by the emergency slaughtered calves, diseased calves then apparently healthy ones (42.9%, 32.7% and 20%, respectively). These isolates were confirmed as Pasteurella multocida microscopically by staining with Gram’s and Leishman’s stains, biochemically by traditional tests and by API 20E commercial kit then by PCR. A total of 221 serum samples were collected: 50 samples from apparently healthy animals, 156 samples from diseased animals and 15 samples from emergency slaughtered animals. The percentage of positive serum samples using micro-agglutination test using somatic antigen were 10%, 54.49% and 0% in apparently healthy, diseased and emergency slaughtered samples respectively at 1/1280 diluted serum. Whereas, the percentage of positive samples using indirect haemagglutination test using capsular antigen were 40%, 60.89% and 60% in apparently healthy, diseased and emergency slaughtered samples respectively at 1/1280 diluted serum. While when applying ELISA using somatic antigen it was observed that the percentage of positive samples were 20%, 35.9% and 46.7% in apparently healthy, diseased and emergency slaughtered samples respectively. Whereas, the ELISA assay using capsular antigen showed positive percentage 42%, 92.9%, 80%. The obtained results revealed that the ELISA using capsular antigen of Pasteurella multocida is a more sensitive and specific serological test for Pasteurella multocida diagnosis.