الفهرس 
IntroductionOnly 14 pages are availabe for public view
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Abstract
Azurin is a low molecular weight protein (128 aa- 14 kDa), member of the cupredoxin family produced by Pseudomonas aeruginosa. Cupredoxins are copper-containing water-soluble proteins involved in the electron transport chain of prokaryotes and eukaryotes. Azurin has anticancer, antiparasitic, antiprotozoa and antiviral properties. These properties are due to azurin’s ability to establish high-affinity interactions with some proteins relevant in these diseases. This ability makes azurin a natural scaffold protein that has immunoglobulin-like binding characteristics. In this study, P. aeruginosa was isolated randomly from a cultured plate of different specimens belongs to patients who attended to inpatients and outpatients clinics at Mansoura University Hospitals from January 2010 to July 2013, and identified manual biochemical tests then confirmed by using Microscan Walk away 90 systems, in Pediatrics Hospital, Microbiology Lab, Mansoura University, about 10% of them able to produce blue green color (azurin) and optimize the favorable broth media for the azurin production. Azurin gene was amplified using the suitable PCR programme. The purification of azurin had been performed by Colum chromatography on superdex 75, then study cytotoxicity effects of crud and purified azurin against MCF-7 cell line. The gene was cloned into pJET 1.2/blunt vector, and genetic modification of the promoter region of the azurin gene by sub cloned into T7 induced vector (pET-21a (+)) construct was transformed into BL21 (DE3) E-coli after digestion by xbal and xhol to improve azurin production and enhance its activity on MCF-7.