الفهرس 
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Abstract
This study was conducted on Raw milk and some types of cheese produced in Egypt (200 samples (Karish, Domiati and Cold stored cheese ”Thalaga”) (50 of each)) to investigate prevalence of S. aureus (enumeration and isolation), detection of genes encoding SEs in isolates by PCR and presence of SEs using ELISA. Staphylococci were detected in 66 %, 66% and 74% samples out of the examined karish, Domiati and thalaga cheese samples respectively, with a mean count of 1.19x107+ 1.59x105, 4.41x106+ 2.05x106 and 5.11x106 + 2.15 X106 cfu/gm, respectively. S. aureus was detected in 42%, 36%, 34% of karish, Domiati and thalaga cheese samples with mean S. aureus count of 8.18 x 104 + 4.51x 105, 2,03 x 103 + 1.14x 103 and 2.43 x 105 + 2.01 X 105 cfu/gm, respectively . Karish cheese extract of 5 samples with S. aureus count ≥105 cfu/gm, all S. aureus positive Domiati and thalaga cheese samples extract undergo Double sandwich ELISA and sowed that 1 sample extract of karish cheese SEA, and 1 sample extract of thalaga cheese expresses SEB. That confirmed by detection of S. aureus enterotoxin gens by PCR, showed that presence of sea in S.aureus of karish cheese isolates, presence of seb in S.aureus of thalaga cheese isolates. That indicate manufacturing of cheese samples seem to be related to the use of mastitic raw milk or unhygienic production, handling and storage that posse a risk for consumers. All measures should be applied to prevent the production of SE during processing and the occurrence of SE in the final product.