الفهرس 
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Abstract
Prolactin (PRL) plays a crucial role in the initiation and maintenance of lactation in animals. It is a potential quantitative trait locus and genetic marker of production traits in dairy cattle. However, no SNP was detected and no association studies was conducted on PRL gene in Egyptian buffalo up till now. Therefore, the objective of this study was to detect PRL gene polymorphisms in Egyptian water buffalo. A PCR product of 245 bp, including part of the promoter of PRL was amplified using PCR and subsequently, SSCP and nucleotide sequencing were conducted to identify its different allelic patterns in Egyptian buffaloes. Our results revealed presence of a novel T72C SNP in the PRL promoter. Two genotypes (TC, CC) were identified using SSCP technique. These genotypes did not showed association with milk traits (milk yield, fat%, protein%, lactose%, solid%) in Egyptian buffaloes. We conclude that, no significant difference in milk traits between Egyptian buffaloes have TC or CC genotypes and so breeders should not depend on this SNP in their selection for dairy animals.
Keywords: Prolactin, Egyptian buffalo, PCR, Sequencing, SNPs