الفهرس 
IntroductionOnly 14 pages are availabe for public view
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Abstract
Sixty eight adult male Wistar rats (200–250g body weight) were housed in cages under a temperature-controlled environment of 24 ± 1 ° C with a 12-hour dark/light cycle (dark cycle: 8: 00 p.m. to 8: 00 a.m.) and housed with free access of food and water throughout the experiments. The animals were divided into five groups used for behavioral and histological studies. (1). Normal control group: age-matched rats (n=16) received no manipulations, served as normal controls. (2). Lesioned group: rats (n=16) were lesioned by ibotenic acid injection in the hippocampus. (3). Sham control group: rats (n=8) were given injection of vehicle (4μl of CSF) in the hippocampus. (4). Sham lesioned group: rats (n=8) were given injection of vehicle (4μl of CSF) in the hippocampus after lesioning by IBO. (5). Adult human olfactory blub derived neural stem cell (OBNSCs) transplantation group (treated group): rats (n=20) received OBNSCs transplantation 10 days after hippocampal lesion. The histopathological, immunohistochemical and behavior analysis were used to evaluate the therapeutic potential of adult human OBNSC and their ability to restore normal memory and cognitive functions at the behavioral and histological levels and there were significant improvement noticed in tissue architecture and behavior of the animals. Conclusion: the present study demonstrated that human OBNSCs expressing NGF ameliorate the cognitive deficiencies associated with ibotenic acid-induced lesions in AD model rat via increased NGF production. These results indicate that human OBNSCs expressing NGF are promising candidates for the cell-based gene therapy for AD patients.