الفهرس | Only 14 pages are availabe for public view |
Abstract A trial was made to isolate Staphylococci from some poultry farms suffering from septicaemia, arthritis and swollen heads. In this study, 217 random samples from joints, swollen heads and various organs were collected from 80 chickens at different ages. • The occurrence of S. aureus and S. epidermidis was 27.05 and 11.76%, respectively. • Incidence of S. aureus as regard to sites and lesions was highest (43.75%) in chickens showed arthritis (joint lesion) followed by swollen head, yolk sac, heart blood, liver in a percentage of 25, 25, 22.58 and 20%, respectively. • Sensitivity of the isolated S. aureus strains indicated that ampicillin followed by amoxicillin, penicillin and lincospectin were the most effective antibiotics. S. aureus strains were sensitive to these antibiotics with a percentage of 50, 45, 35 and 35%, respectively. • The pathogenicity of S. aureus to 5 day-old balady chicks revealed that subcutaneous route of infection gave higher incidence of mortalities than the oral route. • Infective dose of 105 induced clinical signs and postmortem lesions. On the other hand, a dose of 1()6 gave severe clinical signs and severe postmortem picture became more severe. • Plasmid DNA profile of the isolated S. aureus using polyacrylamide gel electrophoresis showed two different size of plasmid DNA (5 and 8 Kbp). |