الفهرس 
IntroductionOnly 14 pages are availabe for public view
 |  | from | 79 |  |  |
| | | from | 79 | | |
Abstract
The purpose of the present study has been to estimate the prevalence of Campylobacter jejuni in fresh dressed marketed chickens as well as its incidence among children diarrhoeal cases and to identify the recovered Campylobacter jejuni. A total of 50 random chicken sanales were obtained from different poulterer butcher shops at Kalyobia Governorate. Samples were taken from skin, caecal content, liver and muscle of each chicken. Fresh stool specimens were collected from a 100 ill children (2 - 5 years old) suffering from diarrhoea (attending patients to the outpatient clinic of the pediatr:ic division of a Governmental hospital). All samples were transferred to laboratory without undue delay and were inoculated on enrichment broth at 42°C for 24 hours; then samples were inoculated onto CBFSA and incubated at 42°C for 48 hours in a microaerobic atmosphere (5% O2 : 10% CO 2 : 85% N 2 ) usirgan anaen:t>ic jar with Campylobact.er Gas Generating Kit (Oxoid BR 56 envelope). Suspected colonies were isolated and subjected to microscopical and biochemical examination as well as biotyping according to Skirrow and Benjamin (1980). Campylobacter jejuni could be isolated from skin, caecal content and liver of chicken samples with 40%. 20% and 16%, respectively. Failure to isolate Campylobacter jejuni from the chicken muscle samples has been discussed. Campylobacter jejuni was isolated from the children stool specimens with 4% incidence. Campylobacter jejuni biotype ”1” was the most predominant Campylobacter jejuni recovered from chicken and children samples. The current investigation discussed the hygienic importance of the Campylobacters organisms and the hazard of implication of these pathogens in outbreaks of food borne illness. Thus. it would perhaps be justified to add Campylobacter jejuni analyses to the routi ne control of poultry products.