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Abstract One hun red and thirty two random samples of ready to eat market edible offals (32 liver and 25 each from spleen, lung, intestine and rumen) were collected from different localites in Assiut as restaurant, street vendors, shelves, and subjected for bacteriological examination and estimation of: 1- Enumeration (count): 1- Total bacterial: Total colony count/gm samples ranged from 5 x 106 to 7 x 108 with an average with an average of 1 x 109; 8 x 107 to 3 x 109 with an average of 1 x 109; 5 x 107 to 2 x 109 with an average of 7.3 x 108; 5 x 107 to 1 x 109 with an average of 5.8 x 108, in examined cooked edible offals liver, spleen, lung, intestine, and rumen respectively. The total colony count/gm. were highest in each of spleen and lung samples, while the lowest count was in liver samples. 2- Enterobacteriaceae: The total Entepobacteriaceae count/gm samples of liver, spleen, lung, intestine and rumen ranged from 1 x 103 to 553 6 6 x 10 with an average of 1.2 x 10 ; 2 x 10 to 5 x 10 with an average average of 2.4 x 106; 1 x 104 0 1 x 107with an average of 1.8 x 106; 1 x 104 to 1 x 1 7 with an average of 9.5 x 105 respectively. The h•~hest total Enterobacteriaceae count was in the lun samples, while the lowest count was in the liver samples. The total enterococci co nt/gm of examined cooked 3- Enterococci: samples from liver, spleen, 1 ng, intestine and rumen ranged from 1 x 103 to 5 x 10 with an average of 6 x 104 ;47.64 7 10 to 2 x 10 wlth an averag of 3.6 x 10 ; 10 to 10 with an average of 106; 104 t 2.7 x 107 with an average of 3 x 106; 10 to 2 x 106 wi h an average of 7 x 105, respectively. The highest en erococci count was in spleen samples followed by intestine lung, rumen, while the lowest count was in liver sam les. 4- MPN of coliforms/gm: The MPN of coliforms/gm of cooked liver samples ranged from 2 x 102 to > 1 x 105, (6’5.63%) were in the range of 1 x 102 _ > 1 x 103 and (34.37%) were in the range of 1 x 104 _ > 1 x 105; Regardiqg to spleen samples the MPN of coliforms/gm ranged from ~03 to > 105 , (76%) of the samples were in the range of 1 x 105 - > 1 x 105 and (24%) were in the range of 103 - < 1 x 105/gm; On the other hands the MPN of coliforms/~ of examined lung samples ranged from 2 x 102 1 x 5 to > 10 /gm, (76%) from the total samples were in the range of x 104 - > 1 x 105 and (24%) were in the range of 2 x 102 < 105; Concerning to the intestine samples, the MPN of coliforms 1 x 105 , (72%) were in the ange of 2 ranged from x 104 - > 1 x and (28%) were in the range a 5 x 10 2 - < 10 4 ; Further- (76%) were in the range of 1 were in the range of 1 x 10 3x 103 -105 _ < 105 /gm. samples revealed that the 5 to ) 1 x 10 /gm, > 1 x 105 and (24%) more examination of cooked ru en MPN of coliforms ranged from The highest MPN of coliforms was in spleen an rumen samples, while the lowest count was in the liver samples. 5- The MPN of E. Coli: The MPN of E. CoZi/gm. samples of liver was the lowest count than other examined organs and ranged from < 3 to > 1 x 105, (62.5%) were in the range of < 3 to 5 x 10 2 ,(37.5%) were in the range of 1 x 103 to > 1 x 105; while the MPN of E. CoZi for all e~amined organs ranged from < 3 to > 1 x 105. Most rrequencv of samples (68%) were in the range of 1 x 105 - > 1 x 105 , (32%) were in the range of < 3 to 1 x 103 in C$.se of spleen samples; on the other hands most lung sample$ (60%) were in the range of > 1 x 104 to > 1 x 105, (40%) were in the range < 3 _ < 104; Regarding to intestine most frequency of samples (56%) were in the rapge were in the range of < 3 to < 10 : Furthermore most frequency of examined rumen samples (68%) were in the range of 2 x 104 to > 1 x 105 , (32%) were in the range of < 3 to 5 x 103. Hence the highest count was in the spleen samples. 11- Isolation 6.1. Staphylococcus aureus coagulase positive Staph. aureus coagulas positive were detected in 50%: 52%: 44%: 48% and 40% from examined liver, spleen, lung, intestine, and rumen respectively. 6.2. Shigella ShigeLLa sp. could be isolated from cooked edible offals, liver, spleen, lung, intestine and rumen as 3.13%: 8%: 8%: 12% and 12% respectively. 7.1. E. Coli Type I (typical) could be detected in 40.6%: 48%: 40%: 52%: 48%, from examined liver,spleen, lung, intestine and rumen samples respectively. Furthermore E. CoLi type II and intermediate could be isolated from all examined samples. 7.2. Other potential tood poisoning organisms: KLebsieLLasp.,. EnteY’:Jbacter sp . , Citrobacter ap . , Arizona sp ,, Proteus mira!:: i i.«, Proteus rettgri could be detected in 9.38%, 9.38%, 15.62%, 31.25%, 15.62%, 9.38%. |