الفهرس | Only 14 pages are availabe for public view |
Abstract The current study aimed to reduce the urinary catheter contamination by biofilm forming Gram negative bacteria, e.g. E. coli, Klebsiella pneumoniae, Proteus mirabilis and Pseudomonas aeruginosa and Gram positive bacteria, e.g. Staphylococcus epidermidis and staphylococcus aureus. Also the study aimed to detect the genes responsible for biofilm formation in different bacteria and suggestion of different coating systems for the urinary catheters to reduce catheter associated urinary tract infections (CAUTI). Among the 128 urinary catheters collected, one hundred and eight (108) were showed adherent bacteria. from these urinary catheters, 150 isolates were obtained and identified as: E. coli 40 isolates (26.7%), 28 isolates (18.7%) as Klebsiella were identified, 20 (13.3%) Proteus isolates, 22 (14.7%) Pseudomonas isolates, S. epidermidis isolates 28 (18.7%) and 12 (8%) isolates S. aureus (Table 6 and Figure 9). Identification of clinical isolates were based mainly on the morphological, culture characteristics and the biochemical tests according to Koneman et al. (1997) and Forbes et al. ( 2007). Biofilm production is an important pathogenic factor which facilitates the adherence of microorganism to medical devices and protects them from the host immune system and antimicrobial therapy. As shown in (table 7 and figure 10), the clinical isolates were classified according to the degree of biofilm formation and adherence into 4 groups which are non, weak, moderate and high producers. In this study, As (table 7 and figure 10) revealed, Gram negative bacteria were more predominant than Gram positive bacteria as highly biofilm producers. Where, 36.4% of Pseudomonas isolates were highly producers followed by E. coli and Proteus isolates (30% each). Klebsiella 125 isolates were the least highly biofilm forming bacteria among Gram negative isolates. |