الفهرس 
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Abstract
The present investigation concerned with the effect of carbon dioxide concentration (greenhouse effect) and degree of salinity on growth of freshwater green micro alga Chiarella vulgraris. It is well known that CO2 and sodium ions essential factors limiting algal growth. Carbon dioxide emISSIOns are produced naturally through the carbon cycle and through human activities like burning of fossil fuels. Natural sources of CO2 occur within the carbon cycle where billions of tons of atmospheric. CO2 are removed from the atmosphere by oceans and growing plants. Carbon dioxide emissions are responsible for about 80% of the problems related to greenhouse gas emissions. Greenhouse gas emissions those greenhouse gases allow sunlight to enter the atmosphere freely and contribute to the greenhouse effect, which many believe is the cause of global warming. The primary greenhouse gases though to be major contributors to global warming are, Carbon dioxide emissions (C02), Methane emissions (CH4), and Nitrogen oxides (N20). Salinity of soil and irrigation water is a serious problem in agriculture leading to severe crop losses. It also considered an important ecological variable in the fresh water and marine envirorunent. Such changes in salinity of water often affect the growth, metabolism and photosynthesis of phytoplanktons. Salt might have a direct effect upon processes involved in electron transport and photophosphorylation and result in decrease in the quantum efficiency of photosynthesis. The objective of this study is to investigate the effect of 3. Opposite vulgaris s dioxide co 4. The effect Chlorella different concentrations of RCO 3 (0.02%, 0.1 % and 0.5%) as well as salt concentrations Na + (0.1 %, 0.5% and 1 %) on firstly: development of the photosynthetic apparatus of unicellular alga Chlorella vulgaris as it expressed as chlorophyll pigment content ()1g ChJOIOphy)]- a+b.109 cell). Secondly: on biochemical composItIOn as it estimated as carbohydrate accumulation (Ilg D-glucose .1 06 cells) and protein content (Ilg protein .1 06 cells), and thirdly: as growth rate of algal cell as they determined by associating cell numbers (106 ml-1) . The approach has been done to conduct experiments under different carbon dioxide or salt concentrations to study the response of Chlorella vulgaris cells toward these stresses under two certain levels of inorganic nitrogen source: standard medium (0.1% KN03) and nitrogen - enriched medium (0.5% KN03). The results of this work can be summarized in the following: 1. Generally the concentration of inorganic nitrogen source appears to be the nutritional factor limiting the growth of Chlorella vulgaris cells followed by carbon dioxide. 2. Accumulation of biochemical constituents as well as developing of photosynthetic apparatus is related in diversity to the concentration of salts in the medium. 3. Opposite to the salt concentration the cells of Chiarella vulgaris show slight increases in the direction of carbon dioxide concentration.4. The effect of carbon dioxide as nutritional limiting factor on Chiarella vulgaris IS influenced greatly by nitrogen concentration, but it absent in the case of salt treatments.5- Due to the high proportion of protein (45%), fat (20%), carbohydrate (20%), minerals and vitamins (10%), and fiber (5%) in Chiarella vulgaris when dried, is therefore recommended for use in fish feeding in fish farms also this food is natural and safety. ts under ponse of in levels 03) and source owth of.