الفهرس 
Histological review of the mucosa of thestomachOnly 14 pages are availabe for public view
 |  | from | 153 |  |  |
| | | from | 153 | | |
Abstract
Indomethacin is a well known non steroidal anti-inflammatory drug.
It exerted many side effects including acute gastritis.
Melatonin is a strong antioxidant. The protective role of melatonin
against indomethacin-induced gastritis was a point of controversy.
Misoprostol is a synthetic prostaglandin (PGE1). Many literatures
stated conflicting observations concerning the prophylactic effect of
misoprostol against indomethacin-induced gastritis.
The purpose of the present study was planned to assess the
prophylactic role of the administration of melatonin, misoprostol or both
versus the indomethacin-induced acute gastritis.
Thirty-five adult male albino rats were fasted for 24 hours before drug
ingestion to assure the full effect and dose of each drug. The rats were
segregated into two major groups: GI: sham control group: further
subdivided into (A, B and C) and G П: indomethacin-treated group:
further subgrouped into (A, B, C and D).
Subgroup IA: received normal saline through gastric gavage tube.
Subgroup IB: received melatonin (60 mg/kg bw) through gastric gavage
tube.
Subgroup IC: received misoprostol (50μg/kg bw) through gastric gavage
tube.
Subgroup IIA: received indomethacin (48mg/kg bw) through gastric
gavage tube.
Subgroup IIB: received indomethacin and melatonin in the preceded
doses through gastric gavage tube.
Subgroup IIC: received indomethacin and misoprostol in the preceded
doses through gastric gavage tube.
Subgroup IID: received indomethacin, melatonin and misoprostol in the
preceded doses through gastric gavage tube.
The rats were sacrificed 4 hours after drug ingestion. The body
region of the stomach was selected and examined by magnifying lens to
calculate the ulcer index then it was examined by the light microscope.
Histomorphometric study was done using the image analyzer computer
system to calculate the mean of the area percentage of the connective
tissue, the mean of the optical density of the PAS reaction and the mean
of the area percent of the stem cells.
The study revealed that indomethacin caused high ulcer index,
extensive destruction of the mucosa, observable lymphocytic infiltration.
There was scanty amount of connective tissue, mucous secretion and
proliferating stem cells.
In the group receiving indomethacin and melatonin, there was
improvement of the morphology of the gastric mucosa in the form of:
significant decrease of the ulcer index, infrequent foci of superficial
erosions surrounded by normal mucosa associated with decrease of the
lymphocytic infiltration. The amount of connective tissue, the volume of
mucous secretion and the number of the proliferating stem cells were
increased.
In the group receiving indomethacin and misoprostol, there was
observable improvement of the morphology of the gastric mucosa in the
form of: high significant decrease of the ulcer index, almost normal
gastric mucosal architecture, increased thickness of the mucous secreting
cell layer. The amount of connective tissue, the volume of mucous
secretion and the number of the proliferating stem cells were markedly
increased.
In the group receiving indomethacin, melatonin and misoprostol,
there was marked improvement of the morphology of the gastric mucosa
more than that observed when administering each drug alone. This was
manifested by: very high significant reduction of the ulcer index and
almost normal gastric mucosal architecture. The amount of connective
tissue, the volume of mucous secretion and the number of the
proliferating stem cells were markedly increased more than the preceding
groups.
The study was supported by statistical analysis which showed
significant results when comparing the different parameters of subgroups
IIB, C and D to subgroup IIA and IA.
In conclusion, indomethacin induces morphological and
histomorphological injuries to the gastric body mucosa of rats. Melatonin
has a partial protective role against indomethacin-induced gastritis,
misoprostol has a more protective effect in indomethacin-induce-gastritis
while using both drugs in combination, resulted in effective protection
against indomethacin -induced gastric lesions more than each drug alone.
Keywords: Indomethacin, Stomach, Melatonin, Misoprostol