الفهرس | Only 14 pages are availabe for public view |
Abstract Renal transplantation is the treatment of choice for patients with end-stage renal disease (ESRD). However, the clinical success of this procedure remains dependent on the lifelong administration of immunosuppressive medications. These agents have a variety of undesirable side effects, many of which are directly related to the agent’s efficacy at suppressing the immune system CD8 is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. In general, the role of the CD8+ T cells is to monitor all the cells of the body, ready to destroy any that express foreign antigen fragments in their class I molecules. CD20 is a non-glycosylated phosphoprotein expressed on the surface of all mature B-cells. This gene encodes a member of the membrane-spanning 4A gene family, it plays an important functional role in B-cell activation, proliferation and differentiation. This study includes 28 selected cases of renal allograft rejection which are received at Cairo University-(Al Kasr Al Aini) pathology department as well as a private lab in the period between 2000 and 2009 were reviewed. Immunohistochemical staining of CD20 and CD8 was done using the streptavidin-biotin technique. This thesis consisted of introduction, aim of work, review of literature, material and methods used, results illustrated by images, discussion of results, conclusions, recommendations and list of references, together with a summary in English and another in Arabic. This work revealed that there was a weak positive correlation between CD20 cells and C4d positivity and positive correlation with CD8 and grades of rejection We found CD8 cells didn’t correlate with any of the acute or chronic scores, with C4d positivity, time post transplantor grade of rejection. Key words: Renal rejection, B lymphocytes, T lymphocytes, CD20 and CD8. |