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Abstract Two expriments were carried out in the exprimental station of Botany Department At Faculty of Agric. Benha Univ. Moshtohor and this was in 2003-20004 season by Using Pinus seeds of the 2 cultivators Pinea and Brutia which were imported from Syria. And Those seeds were treated by growth regulators for improving the seedlings emergence percentage & the growth of those plants. The results of the used treatments were as following : I - control (without a comparison treatment ) 2-Cytokinin treat. ( Benzyle Adenine ) BA by conc. Of 10-20 ppm. 3-Auxin treat. ( Nephthalyne Aceti Acid ) by 2 conc. 25-125 ppm. 4-Gibberellin treat. ( Gibberellic Acid ) by 2 conc. 50-250 ppm. 5-CytOkinin high conc. and Auxin high conc. Interaction treatment (BA at 50 ppm + GA3 at 125ppm) 6-Cytokinin high conc. And Gibberellin high conc. Interactiontreatment (BA at 50 ppm + GA at 250 ppm) 7-Cytokinin high conc. , Gibberellin high conc. and Auxin high copnc. Interaction treatment (BA at 50 ppm + NA A at 125 ppm + GA at 250 ppm) So, Total treatments are 10 treatments for each planted type , and each treatment was divided into 5 replicates, each containing 10 bags So total number of plants for each type is 500 plant. First expriment : Using Pinus seeds of the 2 cultivates Pinea and Brutea and planting them after treatment by growth regulators in the previus treatments by soaking for 24 hrs , then planting in polyethylene bags 1Kg ( sand and clay mixture by 1:1).treatments were divided into replicates each containing 10 bags in 5 replicates ,So totol bags will b 500 bags. For measuring the seedlings emergence percentage d estimating the hormonal content of resulting plants roots and making anatomical sections for each of roots & stems after stabilization o seedlings emergence percentage. Second Expriment : 500 plants were used each of 6 M age and were m aged for another 6 M till the age of 1 y , then using same previous tre • ents for 5 M spraying on the plant starting from 13 M age until 17 age for observing plants successive growth measurements from lY till 2Y age,which were as following : Firstly: Growth measurements from 13-24 M : Plant Height Stem Diameter -Nunber of lateral branches -Number of leaves Secondly: Growth measurements at 30M : 1-stem measurements : Stem Height -Stem Diameter -Number of lateral branches-Length of lateral branch s- Fresh and Dry weights. 2-Leaves measurements: -Number of leaves -Average length of 4 leaves -Fresh &Dry weights. 3- Root measurements: Root diameter (1 cm from the base) —number of late 1 roots —root size —fresh and dry weights Also, dry matter distribution into different plant organs was estimated. Obtained Results can be summarized as following : Firstly: Percentage of seedling emergence and germination rate. The interaction treatment of the applied three growth regulators gave the highest % of seedling emergence that reached 80 % meanwhile it was only 50 % in control. Secondly : Endogenous phytohormones in roots (at 6 months of plant age). * In both cvs.; the treatment of GA3 increased the endogenous IAA in roots of treated plants. *The endogenous gibberellin only increased with GA3 treatment meanwhile, other treatments decreased its level. Also, BAtreatment increased the endogenous levels of auxin and gibberellin. * On the other hand , different applied treatments decreased the endogenous level of abscisic acid in roots. Thirdly: growth measurements at the age of 13 months upto 2 years: 1- Pinus Pinea different applied treatments increased plant height to reach its maximum (63 cm) meanwhile, it was only 74cm in case of control- the same was obtained for stem diameter. * The interaction treatment of BA+GA3 gave the highest number of lateral branches (22 in comparison with branches in control) *Also, for leaves number; the interaction treatment of the three growth regulators gave its maximum followed by GA3 treatment.2- Pinus brutia : All applied treatments increased each of plant height and stem diameter as well For branches number it showed its maximum with interaction treatment of the three applied growth regulators m anwhile, maximum leaves number was obtained with BA+G 3 treatment. Fourthly: Growth measurements at 30 months of plant age. 1-Pinus Pinea : For each of plant height and stem diameter were obviously increased with different combinations applied to reach its maximum with the three° growth regulators combination. *For branches number it reached its maximum that reached 28 branches in comparison with only 19 branches in c e of control treatment . The same trend was also obtained in cas of leaves number as well as each of root diameter and root dry weight as well. 2-Pinus Brutia : nearly the same positive effect of different applied treatments upon measured growth aspects in Pinus Pinea was also obtained in case of brutia cultivars. Fifthly: Dry matter distribution among plant organs: 1-Pinus Pinea : the treatments of GA3 at 250 and 50 ppm gave the highest dry matter accumulation in treated plants that reached 36-65 and 32-65 gms, respectively. In comparison with only 10.76 gms for control treatment. 2-Pinus brutia : different applied treatments increased the accumulation of dry matter specially the combination treatments. |