الفهرس | Only 14 pages are availabe for public view |
Abstract Regeneration from both callus and suspenSIOn cultures of the medicinal plant Cymbopogon schoenanthus subsp. proximus has been carried out. A range (0.5-8 mg/l) 2,4-D for the induction of embryogenic callus from seed cultures showed that 1.0 and 4.0 mg/l 2,4-D gave 90-100% of embryogenic callus contammg mature embryos within three months of cultures. Testing the effect of different sugar types on somatic embryogenesis maltose and sucrose were efficient for embryo induction (X=4.1 and 3.22), maltose, glucose, lactose for embryo maturation (X=7.9, 6.1, 5.38) while cultures induced on galactose, fructose and lactose (X=15.l8, 9.9, 8.9) best helped healthy shoot growth from mature embryos. Higher sucrose concentration (3 - 4%) gave higher induction and early maturation of somatic embryos, while late maturation and gennination was best on 1 % concentration. Higher phosphorus and lower nitrogen concentrations in the culture media all enhanced embryo induction, low NH4N03 enhanced growth and maturation while low KN03 enhanced embryo germination and shoot production. Long term callus cultures (2.5 years) were maintained on 2,4-D (1-8 mg/l) where regeneration of whole plants was highest from cultures maintained on 8 mg/l 2,4-D. Suspension cultures were initiated on 0.5, 1.0 and 2.0 mg/l 2,4-D and plated on 3 different 2,4-D and BA combinations. Mature embryos were highest on 0.5 and 1.0 mg/l 2, 4-D (X=15.8, 17.3), while emergence. |