الفهرس | Only 14 pages are availabe for public view |
Abstract This work was carried out to study the occurrence of antibiotic resistant bacterial infections in farms and veterinary clinics. A total of 791 animal samples including 391 fecal samples and 400 milk samples of different species of animals (Buffaloes, Cows, Sheep and Goat) and 140 human samples including 76 stool samples, 32 nasal swabs and 32 hand swabs were collected. Bacteriological examination of samples revealed that 71 (18.15%) animal fecal samples, 61 (15.25%) animal milk samples and 27 human samples (19.28%) were positive for Staphylococcus aureus and then were tested for their antibiotic susceptibility by agar disc diffusion method and the results showed that the high resistance was recorded to methicillin (90%). Regarding Enterococcus faecalis, 98 (25.06%) animal fecal samples, 63 (15.75%) animal milk samples and 32 human samples (29.6%) were positive and no resistance was recorded to vancomycin. Also, genotyping of methicillin resistant Staphylococcus aureus (MRSA) showed that all isolates confirmed to be staphylococci, 80% were S. aureus and 80% were methicillin resistance carrying mecA gene. The results proved that antibiotic resistant bacteria might pose a public health problem for humans who are in direct contact with contaminated animals. |