الفهرس | Only 14 pages are availabe for public view |
Abstract The present study was undertaken to improve the production of antibiotic, cephalosporin C, which produced by the fungus C. acremonium. Therefore, the following procedures were attempted : 1-Chemical and physical mutagens were used to induce mutants in C. acremonium. 2- The induced mutants were examined for their ability to produce the antibiotic cephalosporin C. 3-The auxotrophic mutants were examined for their nutritional requirements as well as the relationships between antibiotic productivity and requirements. 4-Some of the induced mutants were also tested for their resistance or sensitivity to four different auntifungal antibiotics. 5-Protoplast fusion was also applied for obtaining recombinant strains with high efficiency in producing the antibiotic under study. 6-Chromatography technique was used to study the antibiotic components in some of the induced mutants. |