الفهرس 
DNA Extraction from frozen bloodOnly 14 pages are availabe for public view
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Abstract
Two strains of El-Adely sheep and Ossimi breed were characterized using biochemical
and molecular genetic techniques. Protein banding pattern using SDS and native gel
electrophoresis were carried out to identify the biochemical genetic fingerprint of each El-Adely
sheep strains (HOR and HMY) and Ossimi breed .Meanwhile, randomly amplified polymorphic
DNA (RAPD) technique was also carried out to identify the molecular genetic fingerprint for the
same individuals using seven random primers.
There were some specific bands (markers) on the biochemical and molecular level,
which can differentiate among El-Adely sheep strains and Ossimi breed. The genetic
homogeneity percentages within each of Ossimi breed, El-Adely HOR strain and El-Adely HMY
strain were 53% ,37% and 55% respectively . Similarity value based on SDS-PAGE, Native-
PAGE and RAPD-PCR analysis was the highest between the two strains of El- Adely sheep
(HOR and HMY) strains ,while it was the lowest between Ossimi breed and El-Adely
sheepHOR strain . Also, similarity value was low between El-Adely sheep HMYstrain and
Ossimi breed. The dendrogram was constructed among the studied sheep groups based on SDSPAGE
, Native -PAGE and RAPD analyses and showed that Ossimi breed was distantly
related from both the two strains of El-Adely sheep (HOR and HMY) strains.
The present study revealed that SDS-PAGE, Native-PAGE and RAPD- PCR
techniques could be strongly recommended as useful tools for detecting fingerprint,
determination of polymorphism and establishing the genetic relationships among different sheep
genotypes.
Key words: Sheep, Ossimi breed, El-Adely strains, SDS-PAGE, Native-PAGE, RAPD-PCR,
fingerprint, homogeneity and genetic similarity.