الفهرس 
Diarrhoegenic Escherchia coliOnly 14 pages are availabe for public view
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Abstract
A total of (863) faecal samples were collected from calves (264 samples), cattle (281 samples) and human (318 samples) from some rural areas of Behera and Menofia Governorates. All faecal samples were cultivated on SMAC agar medium to obtain the characteristic colonies of the bacteria; the colonies were examined using VCA to detect STEC. Positive samples were confirmed to be STEC by serotyping and further confirmation was done by using multiplex PCR reaction to determine the type of Stx. The frequencies and percentages of STEC in faecal samples as examined by VCA were 15 (5.68%), 12 (4.27%) and 7 (2.2%), respectively. Among the 15, 12 and 7 positive samples 14 (5.5%), 12 (4.27%) and 6 (1.89%), respectively were confirmed to be STEC by serotyping and multiplex PCR reaction. higher detection rates were noted in younger animals and man, male sex, farms with low levels of hygiene and in warmer seasons of the year. The overall sensitivity of SMAC medium for isolation of STEC was found to be (36.96% whereas VCA remains the ”gold standard” for confirmation of putative STXproducing isolates for its profound sensitivity on Vero cells. The use of Multiplex PCR with specific primers for Stx1, Stx2 and eae genes revealed the presence or absence of these genes in the tested isolates. This provides a further evidence for potential public and veterinary health hazards Investigation of the epidemiological association between the isolated O157 strains from calves, cattle and man using Random amplified polymorphic DNA RAPD-PCR) fingerprinting clarified the high epidemiological relations between the isolates.