الفهرس 
Bacteriology of salmonella
Salmonella infection on daily farmsOnly 14 pages are availabe for public view
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Abstract
Salmonella; a genus of family Enterobacteriaceae, is a primary etiologic agent of
infectious diarrhea. This organism can be pathogenic for both man and animals.
Salmonellosis, the clinical form of Salmonella infection, is a costly disease to dairy
producers on account of mortality, treatment expenses, reduced milk yield, and weight
loss/decreased weight gain within the herd. Infected cattle can be either clinical or
subclinical, shedding Salmonella in their feces. Moreover dairy cattle infected with non-
typhoidal Salmonella spp. can pose a substantial risk to public health.
In addition to its pathogenicity, there has been concern about antimicrobial
resistance in Salmonella, which has lead to failure of treatment for Salmonella and other
bacterial pathogens and reduction of therapeutic options available.
The present study aimed at:
1- Isolation and identification of Salmonella spp. shed in feces of dairy cattle and their
attendants.
2- Identification of their serotypes
3- Determination of their antimicrobial susceptibility patterns.
This study was carried out on 450 dairy cattle, (47) males and (403) females of various age
groups and all their available attendants, from three different dairy farms in Alexandria
suburbs during the period from June 2007 to September 2008.
Questionnaire sheets including all the relevant information were filled for all studied cattle
and their attendants.
The following samples were collected:
-Fecal samples were collected from all dairy animals of the three examined dairy farms
included in this study, where 150 dairy animals had been randomly selected from each
farm and they were further categorized by age groups (50 from each age group) into the
following:-
1- Pre-weaned calves; fed milk (0- <3 months of age).
2- Growing calves & heifers; between weaning and before first calving (2:3 - <24 months
of age).
3- Adult cattle; they had calved once or more times (2: 24 months).
-All animal attendants were asked to provide stool samples.
-All collected samples were kept in an ice box and were transported to the laboratory
within 2 hours. Each fecal sample was subjected to the following:
1. Non selective pre- enrichment using buffered peptone water.
2. Selective enrichment using IT broth and RVS peptone broth.
3. Culturing on selective media; XLD and BS agar plates.
Isolated colonies (pink or reddish color with black centre on XLD and black colonies
with black halo and metallic sheen on BS) were identified morphologically by microscopic
examination and biochemically to verify that they were Salmonella spp