الفهرس 
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Abstract
Avian pathogenic Mycoplasma is of great concern due to their greater economic effect on production of poultry flocks. It cause severe decrease in egg production, poor hatchability in layer flocks and it remain for long period as a chronic respiratory disease (CRO). This study aim at isolation& biochemical identification of mycoplasma from different egg samples, by 12000 samples were collected from eight chicken layer breed eggs (Balady breed eggs, High six breed eggs, brown Hy-line breed eggs, white Hy-line breed eggs, Brown bovans breed eggs, White bovans breed eggs, Lohmman breed eggs and SPF eggs obtained from different chicken layer farms, then 5 samples (Albumin, yolk, total crushed,external swab and internal swab) were collected from each egg and examined for mycoplasmae, Mycoplasma gallisepticum could be isolated from egg yolk and total crushed samples colected from Balady breed eggs, High six breed eggs, brown Hy-line breed eggs, white Hy-line breed eggs, Brown bovans breed eggs, White bovans breed eggs, Lohmman breed eggs and SPF eggs.The previous results were confirmed by peR test. Pathogenicity test was done in order to compare between the pathogenicity effect of the different Mycoplasma gallisepticum isolates of different breed eggs and the pathogenicity effect of a standard MG- R strain on embryonated SPF eggs. The result showed that MG -R strain caused higher embryo mortality. Sensitivity of peR test was done in order to confirm its sensitivity to identify a small numbers of MG in comparison with culture result and the results confirmed sensitivity and specificity of peR test.