الفهرس 
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Abstract
Salmonella spp are one of the major bacterial causes of food borne gastroenteritis. Poultry, meat products and eggs are most commonly identified as food sources responsible for salmonellosis outbreaks. Salmonella in eggs constitute a public health threat as it found on shell eggs, egg products, hens ovaries and internal eggs contents. Various Salmonella species have been isolated from egg shell, egg yolk and albumen.This study aim at Isolation, biochemical identification and serotyping of salmonellae isolated from hen’s eggs, the use of m-PCR in the identification of the Genus Salmonella and different Salmonella serovars. Then detection of the kanamycin resistant gene yafD in the different Salmonella serovars in the egg albumen and lastly detection of the prevalence of Salmonella spp. infected eggs and the percentages of contamination sites within an egg (on egg shell, the yolk and in the albumen).These followed by detection of (yafD) as the results indicated the presence of the gene in (73.9%) in native eggs albumen and (78.9%) in white eggs albumen while only (63.6%) were identified from the brown eggs albumen.The examined samples were from 250 SPF and 250 eggs from commercial native, white and brown eggs. The incidence of salmonellosis among the native eggs was 2.67% while the incidence of salmonellosis among white and brown egg was 0.8% and 0.53% respectively. These results were confirmed by serotyping and m-PCR. Then 250 albumen samples collected from the native eggs (13.6%) were positive for the genus Salmonella while the 250 albumen samples collected the white eggs (9.6%) samples were positive for salmonella and (7.2%) samples from the albumen of the brown eggs were positive for the genus salmonella.This was the first baseline study regarding the prevalence of a zoonotic agent in eggs. The variables that needed to be analysed were the presence or absence of any zoonotic Salmonella (Salmonella spp.) in or outside the egg.