الفهرس | Only 14 pages are availabe for public view |
Abstract Fascioliasis is the disease caused by liver fluke. Sheep and cattle are the most commonly affected, also other farm animals, horses, deer, goats also may be infected by liver fluke; humans are also occasionally infected. The present study was devoted to throw light on impact of fascioliasis among farm animals and man in some endemic foci in Egypt. To achieve this goal, coprological and serological techniques were carried out, as well as to compare between the excretory/ secretory (EIS) and crude antigens in serological diagnosis of fascioliasis by using ELISA. Coprological examination was carried out on 245 fecal samples collected from 78 sheep, 116 cattle and 51 buffaloes for detection of Fasciola egg. Moreover, 5556 stool samples taken from human also for detection of fasciola egg were collected from patients suffering from liver disorder in Giza and Cairo governorates. Serological examination was carried out on serum samples prepared from blood samples obtained from the same coprologically tested farm animals collected from Cairo and Giza abattoirs. CoprologicaI examination concomitant with serological examination on (46) patients in Cairo and Giza. The obtained results indicated that the serological examination is more sensitive than coprological examination, and can detect early infection of fascioliasis, moreover, the EIS antigen is more antigenic and specific than the crude antigen by using ELISA. |