الفهرس | Only 14 pages are availabe for public view |
Abstract Typhoid fever was diagnosed by blood culture in 13 patients (9 males and 4 females) out of 300 blood samples collected from suspected patients. Thirteen Salmonella typhi isolates and three reference strains (090 I, H901, and Ty2) were subjected to molecular characterization. The quality and quantity of Salmonella typhi DNA were checked by amplifying ribosomal DNA genes and by real time PCR based on TaqMan® chemistry. About 300 fg of bacterial DNA (75 bacterial cells or 525 copies of ribosomal DNA genes) were consistently detectable. Three out of the nine RAPD-PCR decamers used to type the sixteen samples of Salmonella typhi generated twelve distinct PCR types. A total of 85 RAPD bands (81 polymorphic bands) were scored. The discriminatory indices of the three primers ranged from to 0.508 to 0.675. By combining the profiles obtained with the primer trio, an excellent discrimination index of 0.942 was attained. RAPD PCR represents a fast method with high potentials in surveillance and epidemiological investigations of Salmonella infections. |