الفهرس 
IntroductionOnly 14 pages are availabe for public view
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Abstract
SUMMARY
The present investigation was lanned to study the
causal of Sore-shin disease of cott the effect of
some physiological, physical and ch factors geverning
the outbreak and control of the dis caused by Rhizoct-
~ lQ orticium)solani,on cotton pl, t s, The findings can
be summarized as follow:-
1- The tested isolate of -R (-Co-rt-ici-um..•. solani caused cotton
Sore-shin disease with different d grees. The percentage
of both pre-and post-emergence ing off increased by
increasing-’den~i ty •
2- Sodium nitrite followed by sodium and casin were
the best for supporting linear and
J- Fructose, maltose and galactose
ear and amount of growth.
4- The optimum temperature for the Ii ear growth and dry weiof
growth.
hi~est linght
was 25°C.
’- The highest pereentage of cotton s ed germination in Petri
dishes was obtained from seeds ex sed to 600e for 10 minutes
(l
6- The highest percentages of healthy survivals were obtained
from seeds exposed to 600e for 5
followed by 500e for 15 min. Com
7- seed exposed to 6aoe for 15
root and seedling length of
• or 700C tor 15 min.
with the control.
e the highest mean of
cted plants, while
8 -
9 -
;10-
= 10 a
exposing to 500C for 5 min ve the highest mean of
root length s , On the other hand.
the highest mean dry weight as obtained for un-infected
plants from exposure 0 600e for 10 min and to
70°C for 10 min of infected lants,
~»onophenols were always les in healthy hypocotyls of
seedling from seeds exposed 0 hot air treatment. Reduction
varied depending on t e temperature and duration
of exposure. Hot air trea ent resulted in a reduc_
tion of monophenol content i the infected hypocotyls
’than control treatment, Polyp enols, increased in all
treatments, in varying degree , compared with the control.
Orthodihydroxy phenols, increased in respone to
infection of plants from seed not exposed to hot air
treatment.
Amino acids, increased in un- nfected plants exposed to
hot air treatments with the xception of few treatments.
Infection resulted in some
henols, in all durations of t
only in 5 min exposure of
Polyphenols, increased in the
ings from seed exposed to hot
infected seedlings showed
different hot air treatments.
of the orthodihydroxyp-
5000 treatment, and
600e and 700e in leaves.
eaves of healthy seedltreatments.
Leaves of
ble responses to the
Monophenols, decreased
~-- -------_._--~-, ._~~~~~-
L5- Seeds soaked in 10 ppm GA3 gave the highest percentage
of seed germination, while NAA at 25 ppm decreased seed
germination percentage in sterilized soil.
16- The highest percentages of healthy survival plants were
obtained from seeds treated with GA3 at 5 ppm, and NAA
a,; 100 ppm.
17- GA
j
at 5 ppm and NAA at 100 ppm reduced root length
18- Growth regulators, reduced the average of dry weight of
healthy plants, while did not affect infected plants.
19 Phenolic compounds in hypocotyls of seeds treated with
growth regulators, decreased in all treatments except
in case of the highest concentrations of both GAJ and
lIJ.A.as response to il~fection, similar to the case of the
•••. ..._ ecids. in ~ec”ted. b.7pocotyl.s were always less
tIIIa tIIId t4 ’UIe CGIIlro1 as a resul.t of treatment with
e ••.~.-tara. elKCept’those receiY1nB the highest
o _~:Lon or .aA, (ZOO ~).
~ ]”a ~_. tIlere -., a pOll~1;:1Yecorre1A1;i.on bet:ween ’the
o.MllL:tat;icm o~ ”-31 or IIAAand. phen*l.1.c cOIIlpound8 i.n
-.J.tiV’ tieaues. ezcaept .anopheu.o1a which showed ’the
., --
III =
contrast!This correlation was noticed in infected
tissues only in case of total phenols. In case of
total free phenols, infection Was accompanied by a
reduction in the ti,ssue content of phenolic compounds
in the respecti ’Ie healthy control
22 - Growth regulators, :Lnduced increase in wnino acids
in healthy tissue in all treatments compared with
the control. On the other hand, those amounts showed
4 vuriation among infected leaves due to the kind
of gro~h regulators and the concentration applied.
a- Soek1ng aeed.ain different concentrations of GA
J
and
Iii tended to increase all sugar fractions of the
healthy hypocotyls ,::omparedwith healthy control. except
GAJ at 5 ppm t:reatments. Infection tended to in-
(~rease the carbob,ydrate content of 1nf’ected. seedlings
1•• ci·]]Z the nao-r••dUC1ng sugar :traction •• The tota~
••I_it. ~ were -ore ’than doubl.ed :Ln the IOO ppm
.:11&&.) - :LDcreaaed !aboat 75%in the 5 PIa GA
3
trea-
.•• • - - - -~~- ~-...- ~~~ .b,;L&1eCJl,i perce-
.~,.
-- Cd .hea1-u..?’ -.a;iw:1.~ p].an:ts (60_42~)_
••. - .JBr-n:~ re ••I1:~ J..:+l &. .a:rop o:r the ’ta1:a1 ADd ::I”J.OEI.-r-ed._ ”’-e -e’ra 1..n ~ :le c~ ~Q ~e UD.-1n%’-
..25 -
..26 -
27 -
’”:~~1.12 =
sugar fractions. Sugar conlent was alway less in the
leaves of infected plants than those of the un-infected
ones at all NAA and GAJlevels except in 5ppm
treatment.
Increasing N and K level led to significant increase
of seed germination percentage while the treatment
of (N2PJK
J
) gave the highest percentage of seed germination.
,;N2PI~ (treatment) gave the highest healthy surVivals
percentage, it was found that a balance between the
three fertilizers (:N”,Pand K) is required to obtain
the highest percentage of healthy survival plants •
P2 .,.-~, NJP2’ NJ~ and NJP2KI treatments gave the highest
average length of un-infected seedlings, while
NI ~ PJ ’ NIP) , NIK) t PJKJ and NrP2KJ treatments gave
the highest average length of infected seedlings
Adding fertilizers at levels of PJ t ~,NJP2t P2KI1
HJK2 and N2PrKr treatments resulted in the highest
dry weight of un-infected seedlings. while B)P2’ NJKJ•
p~ aDd .IP2~ u-ea~-ts gare ”the hishes1; dr:t weight
d i.D:fec1ied. seedJ1Dp.
llr:rP aud K •.1i ~;rer_~ ..-~_ hacl -~ err_’t: •••. ’t~
-tal. and po1ypheno1s in _poco’C7]..s 0% pJI-*_ 1’rGa 1DZ:;
1--
32-
ected and non-infe.Hted soil. For orthodihydroxyphen-
01, infection resuli;ed in a slight decrease in most
treatments. Monophellols increased as a result of infection
As regard amino acids, infection was most accompanied
by a DROP in their amounts when fertilizers were
added.
Monophenols increased in leaves of un-infected plants
in (N) levels, P2,PJ and K) treatments. Infection
reaul ted in a redu.ction in monophenols in the different
levels of N ,Il and K. Similar trend was found for
polyphenols, total free phenols and total amino acids,
w:bich were presen1; at h.J.gher concentration in infected
o:eC\CIIl l.eayea ~l -u. leayes of plants receiving no
JlP8Cifi.c DUtri.~m regi.aes.
Ul %ertiJiser U’l1IB”-Dtsresulted in an :increa.se in
JICT-re’wri uS BUfP’1Ir’S aDd total. soluble sugars of un-
~ec’Hd seecni.” eaept those receiving B1P:rKJ and
JIi’iK) ’Rea- ,,-. ~O’&IU801ub~e sugars and reduoing
-S” were ~ ~ in theun-1nteC’ted plants thaD
’C••• :I.:r 18ge1 1.n ~ecfied cc:m:tiro1. Po1;aasiwa was aeeampa-
D..i.rM by an ~ rollOifed. by a DROP :Ln -the 1;o1;;a.l. ~ea.”Sc 18 ~ ~~ec”,,-a. ---t. ~_’t::ed. b:rpee=
114 =
Increasi g the sup:ply of N,P and K fertilizers exhibited
an nitial rise in reducing sugars followed by
DROP in nfected h,Y’pocotyls.Inc: easing K- level led
tyls. In N and P caused a reduction in total
solublo wl-infected and infected hypocotyls.
to an in rease in sugar fraction and in contrast with
P-levels while N-levels increased and then decreased
sugar co tents.
~; _ Fertili ation treatments led to a reduction in sugar
in un-infected leaves compared with the control.
:fection resul tE~din a DROP in sugar cont ent,
I dir~erent trea~ents of fertilization.
~ - ,tiu._ decrees_ in un-infected hypocotyls reg¥’tl””’!”
.•••• ~ trea~t. Infection caused a reduction in
JIi.~””il level1J1 most treatments. Nutritional trea-
.••••. ” Efl=au..L~ed. in. a aUgb:t DROP o-r Phoaphoroua in
~. ~ec1;ion led to an increase in P-contedJ1~””,,,
ueatments. Potassi\DD was pres-
1IUo6U~r CGl$eeu:tratioDSin lqpocot.Y1s as a res-
~ctri.’t:l••. laud acc•• ula”,ecl to a higher level
••• preseDt 111iDtected cottQD leaves at
tiana hiper ’thaD in ””infected tissues of
plants ceiving s:Lmilar treatments. Non-inoculated
plants ceiving different fertilizers always contained
hi er amounts of Potassium compared with the
non-trea ed control, with no specific trend.