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Abstract
(1)The Pseudomonas areuginosa and Pseudomonas alcaligenes were identified by the Community of Identification of Microorganisms ,Center of Agriculture Search, Cairo, Egypt and also identified according to Bergey ’s Manual of Systematic Bacteriology ; 1984 .
(2)The bacterial isolate Pseudomonas areuginosa was tested for its ability to produce chitinase enzyme in medium containing shell of bolti fish waste and the bacterial isolate Pseudomonas alcaligenes was tested for its ability to produce protease enzyme in medium containing shrimp shell waste.
(3)The fish and crustacean wastes were collected from the fish market in Cairo, Egypt.
(4)The chitinolytic activity was performed using dinitro salicylic acid assay and the proteolytic activity was applied the method given by (Jica, 1995) .
(5)Protein content was followed up applying the method given by ( Lowery et al .,1951 ) . A standard curve of pure bovine serum albumin was constructed and used for this purpose.
(6)Factors effecting chitinase and protease productivity by Pseudomonas areuginosa and Pseudomonas alcaligenes under solid state fermentation conditions were investigated. The following data were found to be optimal for maximal yield of chitinase and protease :
A-An incubation temperature for chitinase enzyme was 37 oC and an incubation temperature for protease enzyme was 40 oC.
B-An incubation period for chitinase enzyme was 48 hour and 30 hour for protease enzyme.
C-An optimum pH for chitinase was 7.0 optimal pH for protease was 7.0.
D-Applying different substrate (shell of bolti fish) concentration on chitinase productivity by Pseudomonas areuginosa, found that 0.215ml is the optimal substrate concentration were the yield of chitinase was increased, and applying different substrate concentration (shrimp shell) on protease productivity by Pseudomonas alcaligenes, found that 0.6/30 ml is the optimal substrate concentration where the yield of protease was increase .
E-An inoclum size for chitinase was 16.5x107 CFU and inoculum size for protease was 20.5x107 CFU .
F-Introducing different nitrogen sources resulted in increasing the yield of chitinase and protease enzymes and reached up its maximum in presence of peptone.
G-Introducing different concentration of yeast extract on the chitinase and Protease were found to be 0.9 g/100 ml is the optimum yeast extract concentration for increase the yield of chitinase and protease.
H-The static state fermentation for chitinase and protease were found to be more favorable than the shaking state.