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Abstract Three new/methods were developed for determlllation of some phenothiaZllle derivatives, spectrophotometrically in bulk and in. different pharme.ceu•~j_eal dosage forms •• Application of one of these methods :f’or analysis of prcme- thazll1e RCl in urine was conducted. The major blue interrA.4QJ) action product c ’_. isolated and identified and a possi- ble reaction mechanism have been suggested 0 Correlation between the optical densities of the produced colour pro due ts with cert;ain phY~ji,cochemical pe.r~\meters of ring substituents were also investigdted • \o.Sh ’~ The thesis cau be a~ . as follows:- Pa”t’t ( 1 ) : •• This :p~t i.ncl’udest’ prelimina.ry testing of 16 phenothia- zine derivatives with 18 amine compounds in addition to 17 oxidants on TLC plates and in f,olutions , in order to choose the most sui ta.ble reagents for quantitative analys~.s of these derivatives. Based on these prelj~lulary investiga- tion, three reagents were selE!cted to pe st’v.diGd ~ ••• Ca) : Morpholine and NHS reagents 0 (b) : Morpholine and iodjne-potassiv~ iodide reage~ts • (c) : Benzocaine and NES reagents 0 Part ( 2 ) :- This part concernes with the development of two new methods for quanti ta”tive analysis of pheno’t_ iazines , using two se’ts of reagents : (a) : Morpholine and NBS reagents, for only C-2 lUlsubstitu- ted phenothiazine derivatives, • (b) : Morpholine and iodine-potassium iodide reagents for 0-2 unsubsti tU.ted a.nd 0””,2 sn:osti 1;uted pnenothiaziiles VIi th cJ values not more than 0023 • The procedures were optimized and applied to thirteen phenothiazine drugs J~ pure f01~ as well as in pharmaceu~ tical formulations. The proposed methods jnvolve the forma.tion of methylene blue like dye stt,ffs in aqueous methanolic m&dium. The developed colou~ed interaction products 1..”’1 all cases show -!;wo prominent absorption peaks. Il’he shorter.A max(s) ranged from 620 - 6L~O run , while the longer ranged :from 660 ~ .• 690 run ~ Beer-’ s law was found be obeyed for all the studied drugs with both reagentz in a wide range of concentrations.. The developed colours were found extremely stable for at least 2Lt hOlU’S • Sensitivity,accuracy, and precision of the proposed procedures were proved by different experiments $ The developed methods were satisfactorily applied for analysis of the studied phenothiazines in the different pharmaceutical dosage ,forms. and morpholine & iod:t.ne”’:po11..> iodide ;reagents .for analysis of promethazine HGl in urin.ecQ The possible mechanism of the involved reactions were , studied using different technic1ues including , TLC ~ sp~c•tro..- photometric sca.nning , IR ,elerr.entarye.nalysi.a as well as chemical testing • Finally correlations of the optical densities of the phenothiazine colotrred interaction products with certain IJhysicochemical parameters (d and ?C) of rir!g substi tuents were investigated • Part ( 3 ) :- This pe~t deals with the use of benzocaine and NBS reaeents for analysis of three phenothiazine drugs ” ~J.lhe , |