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Abstract
The present work was conducted to investigate the clinical and immunological effect of some antifungal feed additives as well as histopathological studies on broilers. Exposure of chicks to OA and the possible protective role of the food additives curcumin and Ms Mycoplus against the fungus (Aspergillus ochraceus) in chicks.
The experiment was carried out on 200, one day old chicks divided equally into 8 groups each one contain 25 chicks.
• The first group was control one, which received the ration without any antifungal feed additives.
• The second group received Ms Mycoplus in the feed.
• The third group taken the curcumin 0.5% in the feed.
• The fourth group received curcumin 1% in the feed.
• The fifth group received Ms Mycoplus with the fungus in the feed.
• The sixth group received curcumin 0.5% with the infection in the feed.
• The seventh group received curcumin 1% with the infection in the feed.
• The eighths group received the fungus alone in the feed.
Hematological and serum biochemical examination and histopathological findings were recorded weekly (2, 4, 6 weeks of age) and the results were compared with those of a normal control group.
The groups treated with the fungus (OA) alone revealed significant decrease in growth rate and body weight gain as well as the groups treated with Ms Mycoplus, while groups treated with curcumin 0.5% showed significant increase in body weight gain followed by curcumin 1%.
Haematological examination revealed microcytic hypochromic anemia and leucopenia in groups treated with the fungus alone and Ms Mycoplus with the fungus. The group that received curcumin 0.5% showed erythrocytosis and leucocytosis, also group which received curcumin 1% showed leucocytosis.
Serum biochemical analysis showed elevation in ALT, AST uric acid and creatinine values, while glucose value did not change. Hypoproteinemia, hypoalbuminemia, hypoalphaglobulinemia and hypogammaglobulinemia were also observed in groups treated with the fungus alone and Ms Mycoplus with the fungus. Cholesterol showed significant decrease in groups treated with the fungus alone and Ms Mycoplus with the fungus
Histopathological examinations showed that OA caused hepatonephrotoxicity, which led to the observed changes in blood parameters in groups treated with the fungus alone and Ms Mycoplus with the fungus. There were significant decease in these changes with the group that received the curcumin 0.5% and 1 %.
Feeding of curcumin 0.5% and curcumin 1% with fungus decreased the toxic effect of OA that produced by the fungus, as observed in the tested parameters.
Conclusion from the result of the present work, it can be concluded that:
1- Mycotoxicosis is one of the dangerous diseases that can result in great losses in poultry production.
2- Antifungal feed additives have a positive role and it must be added to the feed.
3- Using the curcumin as antifungal feed additives (natural) protect the chicks from the negative effect of the fungus.
4- Using the curcumin 0.5% better than curcumin 1%.
5- Using the Ms Mycoplus as antifungal feed additives (synthetic) did not perform the desired effect in compare with the curcumin.
The present work was conducted to investigate the clinical and immunological effect of some antifungal feed additives as well as histopathological studies on broilers. Exposure of chicks to OA and the possible protective role of the food additives curcumin and Ms Mycoplus against the fungus (Aspergillus ochraceus) in chicks.
The experiment was carried out on 200, one day old chicks divided equally into 8 groups each one contain 25 chicks.
• The first group was control one, which received the ration without any antifungal feed additives.
• The second group received Ms Mycoplus in the feed.
• The third group taken the curcumin 0.5% in the feed.
• The fourth group received curcumin 1% in the feed.
• The fifth group received Ms Mycoplus with the fungus in the feed.
• The sixth group received curcumin 0.5% with the infection in the feed.
• The seventh group received curcumin 1% with the infection in the feed.
• The eighths group received the fungus alone in the feed.
Hematological and serum biochemical examination and histopathological findings were recorded weekly (2, 4, 6 weeks of age) and the results were compared with those of a normal control group.
The groups treated with the fungus (OA) alone revealed significant decrease in growth rate and body weight gain as well as the groups treated with Ms Mycoplus, while groups treated with curcumin 0.5% showed significant increase in body weight gain followed by curcumin 1%.
Haematological examination revealed microcytic hypochromic anemia and leucopenia in groups treated with the fungus alone and Ms Mycoplus with the fungus. The group that received curcumin 0.5% showed erythrocytosis and leucocytosis, also group which received curcumin 1% showed leucocytosis.
Serum biochemical analysis showed elevation in ALT, AST uric acid and creatinine values, while glucose value did not change. Hypoproteinemia, hypoalbuminemia, hypoalphaglobulinemia and hypogammaglobulinemia were also observed in groups treated with the fungus alone and Ms Mycoplus with the fungus. Cholesterol showed significant decrease in groups treated with the fungus alone and Ms Mycoplus with the fungus
Histopathological examinations showed that OA caused hepatonephrotoxicity, which led to the observed changes in blood parameters in groups treated with the fungus alone and Ms Mycoplus with the fungus. There were significant decease in these changes with the group that received the curcumin 0.5% and 1 %.
Feeding of curcumin 0.5% and curcumin 1% with fungus decreased the toxic effect of OA that produced by the fungus, as observed in the tested parameters.
Conclusion from the result of the present work, it can be concluded that:
1- Mycotoxicosis is one of the dangerous diseases that can result in great losses in poultry production.
2- Antifungal feed additives have a positive role and it must be added to the feed.
3- Using the curcumin as antifungal feed additives (natural) protect the chicks from the negative effect of the fungus.
4- Using the curcumin 0.5% better than curcumin 1%.
5- Using the Ms Mycoplus as antifungal feed additives (synthetic) did not perform the desired effect in compare with the curcumin.